·binding-characterization
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binding-characterization

adaptyvbio/protein-design-skills

SPR 和 BLI 结合表征实验指南。在以下情况下使用:(1) 规划结合动力学实验,(2) 解决不良/无结合信号的问题,(3) 解释动力学数据伪影,(4) 在 SPR 与 BLI 平台之间进行选择。

14安装·1热度·@adaptyvbio

安装

$npx skills add https://github.com/adaptyvbio/protein-design-skills --skill binding-characterization

SKILL.md

| Sensitivity | Small molecules, fragments (<500 Da) | Large complexes, antibodies | | Throughput | Low-medium (serial) | High (96-well parallel) | | Sample purity | Required (clogs fluidics) | Tolerates crude lysates | | Kinetic resolution | Higher (better for fast kinetics) | Lower | | Mass transport | More sensitive (may distort kon) | Less sensitive |

| Maintenance | High (fluidics system) | Low (dip-and-read) | | Sample consumption | Higher (continuous flow) | Lower | | Cost per experiment | Lower chip cost, higher run cost | Higher tip cost, lower run cost |

| Hydrophobic CDRs | Adsorb to SPR gold/dextran surface | Add 0.05% Tween-20, use CM7 chip with longer dextran | | Aggregation | Mass transport artifacts in SPR fluidics | Filter sample (0.22μm), reduce ligand density | | High instability | Degrades during continuous flow | Shorter cycle time, add stabilizers (trehalose 5%) |

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可引用信息

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安装命令
npx skills add https://github.com/adaptyvbio/protein-design-skills --skill binding-characterization
分类
{}数据分析
认证
收录时间
2026-02-01
更新时间
2026-02-18

快速解答

什么是 binding-characterization?

SPR 和 BLI 结合表征实验指南。在以下情况下使用:(1) 规划结合动力学实验,(2) 解决不良/无结合信号的问题,(3) 解释动力学数据伪影,(4) 在 SPR 与 BLI 平台之间进行选择。 来源:adaptyvbio/protein-design-skills。

如何安装 binding-characterization?

打开你的终端或命令行工具(如 Terminal、iTerm、Windows Terminal 等) 复制并运行以下命令:npx skills add https://github.com/adaptyvbio/protein-design-skills --skill binding-characterization 安装完成后,技能将自动配置到你的 AI 编程环境中,可以在 Claude Code 或 Cursor 中使用

这个 Skill 的源码在哪?

https://github.com/adaptyvbio/protein-design-skills

详情

分类
{}数据分析
来源
skills.sh
收录时间
2026-02-01