binding-characterization
✓Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.
Installation
SKILL.md
| Sensitivity | Small molecules, fragments (<500 Da) | Large complexes, antibodies | | Throughput | Low-medium (serial) | High (96-well parallel) | | Sample purity | Required (clogs fluidics) | Tolerates crude lysates | | Kinetic resolution | Higher (better for fast kinetics) | Lower | | Mass transport | More sensitive (may distort kon) | Less sensitive |
| Maintenance | High (fluidics system) | Low (dip-and-read) | | Sample consumption | Higher (continuous flow) | Lower | | Cost per experiment | Lower chip cost, higher run cost | Higher tip cost, lower run cost |
| Hydrophobic CDRs | Adsorb to SPR gold/dextran surface | Add 0.05% Tween-20, use CM7 chip with longer dextran | | Aggregation | Mass transport artifacts in SPR fluidics | Filter sample (0.22μm), reduce ligand density | | High instability | Degrades during continuous flow | Shorter cycle time, add stabilizers (trehalose 5%) |
Facts (cite-ready)
Stable fields and commands for AI/search citations.
- Install command
npx skills add https://github.com/adaptyvbio/protein-design-skills --skill binding-characterization- Category
- {}Data Analysis
- Verified
- ✓
- First Seen
- 2026-02-01
- Updated
- 2026-02-18
Quick answers
What is binding-characterization?
Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms. Source: adaptyvbio/protein-design-skills.
How do I install binding-characterization?
Open your terminal or command line tool (Terminal, iTerm, Windows Terminal, etc.) Copy and run this command: npx skills add https://github.com/adaptyvbio/protein-design-skills --skill binding-characterization Once installed, the skill will be automatically configured in your AI coding environment and ready to use in Claude Code or Cursor
Where is the source repository?
https://github.com/adaptyvbio/protein-design-skills
Details
- Category
- {}Data Analysis
- Source
- skills.sh
- First Seen
- 2026-02-01